We unclogged the column, but since it was clogging it up for each sample and it’s quite damaging to the column, we decided to stop. Our budget was quite tight, since this was a school project.
All HPLC samples that i do, before putting them on column i filter them through short layer of silica to separate all the insoluble and entirely too polar crap
Additionally there’s also short guard column before main column, so if something goes really fucking wrong it’s the 2cm guard column that is to be replaced and not the 20cm main one
I work mostly with NP-HPLC so your situation might be a bit different, but the general principle is there
did you unclog the column or does lab has to eat up few thousand dollar loss
We unclogged the column, but since it was clogging it up for each sample and it’s quite damaging to the column, we decided to stop. Our budget was quite tight, since this was a school project.
jeez dude are you using some pretreatment
Idk what pretreatment really entails, but we did dilute it, if that counts 😅
All HPLC samples that i do, before putting them on column i filter them through short layer of silica to separate all the insoluble and entirely too polar crap
Additionally there’s also short guard column before main column, so if something goes really fucking wrong it’s the 2cm guard column that is to be replaced and not the 20cm main one
I work mostly with NP-HPLC so your situation might be a bit different, but the general principle is there